Early inhibition of IL-1β expression by IFN-γ is mediated by impaired binding of NF-κB to the IL-1β promoter but is independent of nitric oxide.

Reactive oxygen species mediate TNF-α-induced inflammatory response in bone marrow mesenchymal cells

Objective(s): It is generally believed that the inflammatory response in bone marrow mesenchymal stem cells (BMSCs) transplantation leads to poor survival and unsatisfactory effects, and is mainly mediated by cytokines, including interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α). In this study, we explored the mechanisms underlying the TNF-α-induced inflammatory ...

Endotoxin-mediated nitric oxide synthesis inhibits IL-1β gene transcription in ANA-1 murine macrophages.

On the basis of previous work demonstrating nitric oxide (NO)-mediated inhibition of nuclear factor-κB (NF-κB) DNA binding, we hypothesized that NO downregulates NF-κB-dependent interleukin-1β (IL-1β) production in an ANA-1 macrophage model of lipopolysaccharide (LPS) stimulation. In the presence of LPS (100 ng/ml), levels of IL-1β protein and mRNA were significantly upregulated with NO synthas...

L-arginine attenuates Interleukin-1β (IL-1β) induced Nuclear Factor Kappa-Beta (NF-κB) activation in Caco-2 cells

BACKGROUND Specific nutrients like L-arginine (L-Arg) ameliorate intestinal inflammation, however the exact mechanisms of this effect are unclear. We hypothesized the anti-inflammatory effects of L-Arg require active transport and metabolism by inducible nitric oxide synthase (iNOS) to generate nitric oxide (NO). To test this hypothesis we examined the effects of L-Arg, L-Arg transport activity...

Early Inhibition of IL-1b Expression by IFN-g Is Mediated by Impaired Binding of NF-kB to the IL-1b Promoter but Is Independent of Nitric Oxide

Endothelial PPAR-γ provides vascular protection from IL-1β-induced oxidative stress.

Loss of peroxisome proliferator-activated receptor (PPAR)-γ function in the vascular endothelium enhances atherosclerosis and NF-κB target gene expression in high-fat diet-fed apolipoprotein E-deficient mice. The mechanisms by which endothelial PPAR-γ regulates inflammatory responses and protects against atherosclerosis remain unclear. To assess functional interactions between PPAR-γ and inflam...